Application
Western blot analysis of IKKg in HeLa whole cell lysate with Anti-IKKg (Product No. ) at 1 μg/ml.| Product Name | IKK gamma antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to IKK gammaNuclear factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kB is associated with IkB proteins in the cell cytoplasm, which inhibit NF-kB activity. The IkB kinase (IKKa and IKKb)phosphorylates IkB and mediates NF-kB activation. A novel molecule in the IKK complex was recently identified and termed IKKg/NEMO/FIP3/IKKAP1 (1-5). IKKg interacts with IKKb and is required for the activation of IKK complex and NF-kB by LPS, PMA, TNF, and IL-1 stimulation (1-4). FIP3 was also shown to bind to RIP and NIK and to mediate TNF-induced NF-kB activation (3). |
| Immunogen | Peptide: amino acids 400-416 of human IKK gamma. This sequence is identical to that of mouse. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB: Use at a dilution of 1:500-1:1,000. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AMCBX1 FIP-3 FIP3 Fip3p IKK-gamma IP IP1 IP2 IPD2 NEMO FIP 3 IKK gamma IKKgamma |
|---|---|
| Target | IKK gamma |
| Tissue Specificity | IKK gamma - Does not cross react with IKK alpha, beta or epsilon |
Application
Western blot analysis of IKKg in HeLa whole cell lysate with Anti-IKKg (Product No. ) at 1 μg/ml.| Positive Control | Hela whole cell lysate |
|---|---|
| Application Notes | For WB: Use at a dilution of 1:500-1:1,000. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4 containing 0.02% sodium azide |
