Application
Western blot analysis of extracts of BT474 cell lines, using IKZF1 antibody.Application
Immunohistochemistry of paraffin-embedded rat spleen using IKZF1 antibody at dilution of 1:100 (x400 lens).Application
Immunohistochemistry of paraffin-embedded human kidney using IKZF1 antibody at dilution of 1:100 (x400 lens).Application
Immunohistochemistry of paraffin-embedded human kidney cancer using IKZF1 antibody at dilution of 1:100 (x400 lens).Application
Immunohistochemistry of paraffin-embedded human gastric cancer using IKZF1 antibody at dilution of 1:100 (x400 lens).Application
Immunofluorescence analysis of U2OS cell using IKZF1 antibody. Blue: DAPI for nuclear staining.| Product Name | IKZF1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IKZF1This gene encodes a transcription factor that belongs to the family of zinc-finger DNA binding proteins associated with chromatin remodeling. The expression of this protein is restricted to the fetal and adult hemo-lymphopoietic system, and it functions as a regulator of lymphocyte differentiation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. All isoforms share a common C-terminal domain, which contains two zinc finger motifs that are required for hetero- or homodimerization, and for interactions with other proteins. The isoforms, however, differ in the number of N-terminal zinc finger motifs that bind DNA and contain the nuclear localization signal, resulting in members with and without DNA-binding properties. Only few isoforms contain the requisite three or more N-terminal zinc motifs that confer high affinity binding to a specific core DNA sequence element in the promoters of target genes. The non-DNA-binding isofor |
| Immunogen | Recombinant protein of human IKZF1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PWB |
| WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | IKAROS IKAROS family zinc finger 1 hIk1 Hs.54452 ZNFN1A1 PRO0758 IK1 LYF1 |
|---|---|
| Target | IKZF1 |
Application
Western blot analysis of extracts of BT474 cell lines, using IKZF1 antibody.
Application
Immunohistochemistry of paraffin-embedded rat spleen using IKZF1 antibody at dilution of 1:100 (x400 lens).
Application
Immunohistochemistry of paraffin-embedded human kidney using IKZF1 antibody at dilution of 1:100 (x400 lens).
Application
Immunohistochemistry of paraffin-embedded human kidney cancer using IKZF1 antibody at dilution of 1:100 (x400 lens).
Application
Immunohistochemistry of paraffin-embedded human gastric cancer using IKZF1 antibody at dilution of 1:100 (x400 lens).
Application
Immunofluorescence analysis of U2OS cell using IKZF1 antibody. Blue: DAPI for nuclear staining.| Application Notes | WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
