Application
Cross-linked ChIP was performed with Jurkat chromatin extract and 5 μg of either control rabbit IgG or anti-IKZF1 antibody. The precipitated DNA was detected by PCR with primer set targeting to VPAC-1 receptor promoter.Application
IKZF1 antibody immunoprecipitates IKZF1 protein in IP experiments. IP samples: Raji whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of IKZF1 protein by 4 μg IKZF1 antibody 7.5 % SDS-PAGE The immunoprecipitated IKZF1 protein was detected by IKZF1 antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]Application
IKZF1 antibody detects IKZF1 protein by western blot analysis. A. 50 µg Jurkat whole cell lysate/extract B. 50 µg Raji whole cell lysate/extract C. 50 µg NCI-H929 whole cell lysate/extract 7.5% SDS-PAGE IKZF1 antibody dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | IKZF1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IKZF1 (IKAROS family zinc finger 1 (Ikaros))Transcription regulator of hematopoietic cell differentiation. Binds gamma-satellite DNA. Binds with higher affinity to gamma satellite A. Plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Repressor of the TDT (terminal deoxynucleotidyltransferase) gene during thymocyte differentiation. Regulates transcription through association with both HDAC-dependent and HDAC-independent complexes. Targets the 2 chromatin-remodeling complexes, NuRD and BAF (SWI/SNF), in a single complex (PYR complex), to the beta-globin locus in adult erythrocytes. Increases normal apoptosis in adult erythroid cells. Confers early temporal competence to retinal progenitor cells (RPCs). |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human IKZF1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWBChIP |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Chicken, BovineMouse (94%), Chicken (81%), Bovine (80%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Hs.54452 PRO0758 hIk-1 IK1 IKAROS family zinc finger 1 (Ikaros) IKAROS LYF1 ZNFN1A1 |
|---|---|
| Target | IKZF1 |
| Molecular Weight(MW) | 58 kDa (note) (5) |
| Cellular Localization | Nucleus |
Application
Cross-linked ChIP was performed with Jurkat chromatin extract and 5 μg of either control rabbit IgG or anti-IKZF1 antibody. The precipitated DNA was detected by PCR with primer set targeting to VPAC-1 receptor promoter.
Application
IKZF1 antibody immunoprecipitates IKZF1 protein in IP experiments. IP samples: Raji whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of IKZF1 protein by 4 μg IKZF1 antibody 7.5 % SDS-PAGE The immunoprecipitated IKZF1 protein was detected by IKZF1 antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
Application
IKZF1 antibody detects IKZF1 protein by western blot analysis. A. 50 µg Jurkat whole cell lysate/extract B. 50 µg Raji whole cell lysate/extract C. 50 µg NCI-H929 whole cell lysate/extract 7.5% SDS-PAGE IKZF1 antibody dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | Jurkat , Raji , NCI-H929 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
