Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using IL1 alpha antibody at 1:250 dilution.Application
Sample (30 ug of whole cell lysate) A: HepG2 12% SDS PAGE diluted at 1:1000Application
IL1 alpha antibody detects IL1 alpha protein at cytoplasm by immunofluorescent analysis. Sample: H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IL1 alpha protein stained by IL1 alpha antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | IL1 alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IL1 alpha (interleukin-1 alpha)The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human IL1 alpha. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Rhesus MonkeyRhesus Monkey (89%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | IL-1A antibody IL1 antibody IL1-ALPHA antibody IL1F1 antibody IL1A antibody pro-interleukin-1-alpha antibody IL-1 alpha antibody interleukin-1 alpha antibody preinterleukin 1 alpha antibody hematopoietin-1 antibody "interleukin 1 alpha antibody" |
|---|---|
| Molecular Weight(MW) | 31 kDa (note) |
| Cellular Localization | Secreted |
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using IL1 alpha antibody at 1:250 dilution.
Application
Sample (30 ug of whole cell lysate) A: HepG2 12% SDS PAGE diluted at 1:1000
Application
IL1 alpha antibody detects IL1 alpha protein at cytoplasm by immunofluorescent analysis. Sample: H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IL1 alpha protein stained by IL1 alpha antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | HepG2 |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
