Application
IHC-P analysis of human breast invasive ductal carcinoma using IL17 antibody at a concentration of 0.25 μg/ml. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.| Product Name | IL17 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IL17The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. [provided by RefSeq, Jul 2008] |
| Immunogen | Recombinant Human IL-17 (IL-17A) |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PNeutWB |
| Recommended Starting Dilutions: For ELISA: Use at a concentration of 0.5 - 2.0 μg/ml. For IHC-P: Use at a concentration of 0.25 µg/ml. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. For WB: Use at a concentration of 0.1 - 0.2 µg/ml. For Neutralization: one-half maximal inhibition [ND50] of the biological activity of hIL-17A (50.0 ng/ml), a concentration of 0.9-1.3 μg/ml of this antibody is required. Not yet tested in other applications. Optimal dilutions should be determined experimentally by the researcher.: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | IL17A CTLA8 IL-17A interleukin 17A IL-17 |
|---|---|
| Target | IL17 |
Application
IHC-P analysis of human breast invasive ductal carcinoma using IL17 antibody at a concentration of 0.25 μg/ml. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.| Application Notes | Recommended Starting Dilutions: For ELISA: Use at a concentration of 0.5 - 2.0 μg/ml. For IHC-P: Use at a concentration of 0.25 µg/ml. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. For WB: Use at a concentration of 0.1 - 0.2 µg/ml. For Neutralization: one-half maximal inhibition [ND50] of the biological activity of hIL-17A (50.0 ng/ml), a concentration of 0.9-1.3 μg/ml of this antibody is required. Not yet tested in other applications. Optimal dilutions should be determined experimentally by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS, pH 7.2 |
