Application
Immunofluorescence analysis of methanol-fixed HeLa, using IL1F7 antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using IL1F7 antibody at 1:250 dilution.Application
Sample (30 µg of whole cell lysate) A: A549 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | IL1F7 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IL1F7 (interleukin 1 family, member 7 (zeta))The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine can bind to, and may be a ligand for interleukin 18 receptor (IL18R1/IL-1Rrp). This cytokine also binds to interleukin 18 binding protein (IL18BP), an inhibitory binding protein of interleukin 18 (IL18), and subsequently forms a complex with IL18 receptor beta subunit, and through which it inhibits the activity of IL18. This gene along with eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. Five alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human IL1F7. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FIL1 antibody FIL1(ZETA) antibody FIL1Z antibody IL-1F7 antibody IL-1H4 antibody IL-1RP1 antibody IL1H4 antibody IL1RP1 antibody IL1F7 antibody interleukin-1 homolog 4 antibody interleukin-1 superfamily z antibody interleukin-23 antibody IL1F7 |
|---|---|
| Molecular Weight(MW) | 24 kDa (note) (5) |
| Cellular Localization | Secreted |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using IL1F7 antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using IL1F7 antibody at 1:250 dilution.
Application
Sample (30 µg of whole cell lysate) A: A549 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | A549 , HeLa , HepG2 , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
