Application
IHC analysis of colchicine injected mouse brain (cingulate cortex) tissue using IL6 antibody at a concentration of 0.5 μg/ml. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent.| Product Name | IL6 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IL6 |
| Immunogen | Recombinant Murine IL-6 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-FNeutWB |
| We recommend the following starting dilutions: For WB: Use at a concentration of 0.1-0.2 µg/ml. For ELISA: Use at a concentration of 0.5 - 2.0 μg/ml. For IHC-Fr: Use at a concentration of 0.5 μg/ml. For Neutralization: one-half maximal inhibition [ND50] of the biological activity of Mouse IL-6 (3 pg/ml), a concentration of 0.24-0.36 µg/ml of this antibody is required. Optimal dilutions should be determined experimentally by the end user.: |
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| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Gene Synonyms | Il-6 interleukin 6 Il6 |
|---|---|
| Target | IL6 |
Application
IHC analysis of colchicine injected mouse brain (cingulate cortex) tissue using IL6 antibody at a concentration of 0.5 μg/ml. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent.| Application Notes | We recommend the following starting dilutions: For WB: Use at a concentration of 0.1-0.2 µg/ml. For ELISA: Use at a concentration of 0.5 - 2.0 μg/ml. For IHC-Fr: Use at a concentration of 0.5 μg/ml. For Neutralization: one-half maximal inhibition [ND50] of the biological activity of Mouse IL-6 (3 pg/ml), a concentration of 0.24-0.36 µg/ml of this antibody is required. Optimal dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS, pH 7.2 |
