Application
Immunohistochemical analysis of paraffin-embedded CLEAR CELL OVCA xenograft, using Inhibin beta A antibody at 1:500 dilution.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Inhibin beta-A antibody at 1:200 dilution.Application
Sample(30 µg whole cell lysate) A:H1299 B:HeLa S3 C:Hep G2 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Inhibin beta A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Inhibin beta A (inhibin, beta A)The inhibin beta A subunit joins the alpha subunit to form a pituitary FSH secretion inhibitor. Inhibin has been shown to regulate gonadal stromal cell proliferation negatively and to have tumor-suppressor activity. In addition, serum levels of inhibin have been shown to reflect the size of granulosa-cell tumors and can therefore be used as a marker for primary as well as recurrent disease. Because expression in gonadal and various extragonadal tissues may vary severalfold in a tissue-specific fashion, it is proposed that inhibin may be both a growth/differentiation factor and a hormone. Furthermore, the beta A subunit forms a homodimer, activin A, and also joins with a beta B subunit to form a heterodimer, activin AB, both of which stimulate FSH secretion. Finally, it has been shown that the beta A subunit mRNA is identical to the erythroid differentiation factor subunit mRNA and that only one gene for this mRNA exists in the human genome. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Inhibin beta A. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Cat, Pig, Rabbit, Chicken, Sheep, BovineMouse (100%), Rat (100%), Cat (100%), Pig (100%), Rabbit (100%), Chicken (100%), Sheep (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | EDF antibody FRP antibody INHBA antibody inhibin beta A chain antibody erythroid differentiation factor antibody follicle-stimulating hormone-releasing protein antibody "inhibin beta A (activin A activin AB alpha polypeptide) antibody" FSH-releasi |
|---|---|
| Molecular Weight(MW) | 47 kDa (note) |
| Cellular Localization | Secreted |
Application
Immunohistochemical analysis of paraffin-embedded CLEAR CELL OVCA xenograft, using Inhibin beta A antibody at 1:500 dilution.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Inhibin beta-A antibody at 1:200 dilution.
Application
Sample(30 µg whole cell lysate) A:H1299 B:HeLa S3 C:Hep G2 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
