Application
iNOS antibody detects iNOS protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: iNOS protein stained by iNOS antibody diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.Application
iNOS antibody detects iNOS protein at cytoplasm by immunofluorescent analysis. Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: iNOS protein stained by iNOS antibody diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.| Product Name | iNOS antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to iNOS (nitric oxide synthase 2, inducible)Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse iNOS. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IF |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | MouseMouse (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Nos2a Nos-2 NOS-II i-NOS nitric oxide synthase 2 inducible Nos2 |
|---|---|
| Target | iNOS |
| Molecular Weight(MW) | 131 kDa (note) |
Application
iNOS antibody detects iNOS protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: iNOS protein stained by iNOS antibody diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.
Application
iNOS antibody detects iNOS protein at cytoplasm by immunofluorescent analysis. Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: iNOS protein stained by iNOS antibody diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
