Application
iNOS antibody detects iNOS protein by western blot analysis. Un-treated (-) and treated (+, 50ng/ml LPS treatment for 4hr) Raw264.7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with iNOS antibody at a dilution of 1:1000.Application
iNOS antibody detects iNOS protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. iNOS antibody diluted at 1:500.| Product Name | iNOS antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to iNOS (nitric oxide synthase 2, inducible)Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human iNOS. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Nos-2 Nos2 i-NOS nitric oxide synthase 2 inducible Nos2a NOS-II |
|---|---|
| Target | iNOS |
| Molecular Weight(MW) | 131 kDa (note) (3) |
Application
iNOS antibody detects iNOS protein by western blot analysis. Un-treated (-) and treated (+, 50ng/ml LPS treatment for 4hr) Raw264.7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with iNOS antibody at a dilution of 1:1000.
Application
iNOS antibody detects iNOS protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. iNOS antibody diluted at 1:500.| Positive Control | Raw264.7 (50ng/ml LPS treatment for 4hr) |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
