Application
IHC-P analysis of human liver tissue using INSIG1 antibody at 10μg/ml.Application
WB analysis of human fetal spleen tissue using INSIG1 antibody at 0.2-1μg/ml.Application
WB analysis of human putamen and rat cortex tissue using INSIG1 antibody at 1:1000. Lane 1: human putamen lysate (50ug) Lane 2: rat cortex lysate (50ug)| Product Name | INSIG1 antibody, Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to INSIG1Oxysterols regulate cholesterol homeostasis through the liver X receptor (LXR)- and sterol regulatory element-binding protein (SREBP)-mediated signaling pathways. This gene is an insulin-induced gene. It encodes an endoplasmic reticulum (ER) membrane protein that plays a critical role in regulating cholesterol concentrations in cells. This protein binds to the sterol-sensing domains of SREBP cleavage-activating protein (SCAP) and HMG CoA reductase, and is essential for the sterol-mediated trafficking of the two proteins. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jun 2009] |
| Immunogen | A synthetic peptide corresponding to an Internal region of Human INSIG1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanRat |
| Predicted Reactivity | Cow: 100%; Dog: 100%; Goat: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Sheep: 100%; Zebrafish: 93% |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | INSIG1 CL6 |
|---|---|
| Target | INSIG1 |
| Molecular Weight(MW) | 30 KDa (note) |
Application
IHC-P analysis of human liver tissue using INSIG1 antibody at 10μg/ml.
Application
WB analysis of human fetal spleen tissue using INSIG1 antibody at 0.2-1μg/ml.
Application
WB analysis of human putamen and rat cortex tissue using INSIG1 antibody at 1:1000. Lane 1: human putamen lysate (50ug) Lane 2: rat cortex lysate (50ug)| Positive Control | human fetal spleen , human putamen , rat cortex |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. For short-term storage, store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
