Application
Integrin alpha 5 antibody detects Integrin alpha 5 protein by western blot analysis. Whole cell extracts and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin alpha 5 antibody diluted at 1:5000.| Product Name | Integrin alpha 5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Integrin alpha 5 (integrin, alpha 5 (fibronectin receptor, alpha polypeptide))The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human Integrin alpha 5. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (87%), Rat (84%), Bovine (87%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | VLA5A FNRA ITGA5 integrin alpha 5 (fibronectin receptor alpha polypeptide) CD49e |
|---|---|
| Target | Integrin alpha 5 |
| Molecular Weight(MW) | 115 kDa (note) |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
Application
Integrin alpha 5 antibody detects Integrin alpha 5 protein by western blot analysis. Whole cell extracts and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin alpha 5 antibody diluted at 1:5000.| Positive Control | MDA-MB-231 , MDA-MB-231 membrane fraction extract |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
