Application
Sample (30 µg of whole cell lysate) A: RD (Rhabdomyosarcoma) cell (Mock control) B: RD transfected scramble siRNA C: RD transfected ITGAV siRNA 8% SDS PAGE diluted at 1:900 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Integrin alpha V antibody at 1:250 dilution.Application
Sample (30 µg of whole cell lysate) A: HUVEC 7.5% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Integrin alpha V antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Integrin alpha V (integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51))ITAGV encodes integrin alpha chain V. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. Among the known associating beta chains (beta chains 1,3,5,6, and 8; 'ITGB1', 'ITGB3', 'ITGB5', 'ITGB6', and 'ITGB8'), each can interact with extracellular matrix ligands; the alpha V beta 3 integrin, perhaps the most studied of these, is referred to as the Vitronectin receptor (VNR). In addition to adhesion, many integrins are known to facilitate signal transduction. Alternative splicing results in multiple transcript variants. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human Integrin alpha V. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Pig, Sheep, Rhesus Monkey, BovinePig (92%), Sheep (90%), Rhesus Monkey (99%), Bovine (91%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CD51 antibody DKFZp686A08142 antibody MSK8 antibody VNRA antibody ITGAV antibody integrin alpha-V antibody antigen identified by monoclonal antibody L230 antibody vitronectin receptor subunit alpha antibody "integrin alpha V (vitronectin receptor |
|---|---|
| Molecular Weight(MW) | 116 kDa (note) (3) |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
Application
Sample (30 µg of whole cell lysate) A: RD (Rhabdomyosarcoma) cell (Mock control) B: RD transfected scramble siRNA C: RD transfected ITGAV siRNA 8% SDS PAGE diluted at 1:900 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Integrin alpha V antibody at 1:250 dilution.
Application
Sample (30 µg of whole cell lysate) A: HUVEC 7.5% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | RD (Rhabdomyosarcoma) cell (Mock control) , RD transfected scramble siRNA , HUVEC |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
