Application
Wild-type (WT) and Integrin beta 1 / CD29 knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.Application
Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein by western blot analysis. A. 30 µg A431 whole cell lysate/extract B. 30 µg HeLa whole cell lysate/extract 7.5% SDS-PAGE Integrin beta 1 / CD29 antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein by western blot analysis. A. 30 µg U87-MG whole cell lysate/extract B. 30 µg SK-N-SH whole cell lysate/extract C. 30 µg IMR32 whole cell lysate/extract D. 30 µg SK-N-AS whole cell lysate/extract 7.5% SDS-PAGE Integrin beta 1 / CD29 antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein on breast cancer by immunohistochemical analysis. Sample: Paraffin-embedded breast cancer. Integrin beta 1 / CD29 antibody dilution: 1:500.Application
Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein on hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. Integrin beta 1 / CD29 antibody dilution: 1:500.Application
Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract 7.5% SDS-PAGE Integrin beta 1 / CD29 antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein by western blot analysis. A. 30 µg NIH-3T3 whole cell lysate/extract B. 30 µg BCL-1 whole cell lysate/extract C. 30 µg Raw264.7 whole cell lysate/extract D. 30 µg C2C12 whole cell lysate/extract 7.5% SDS-PAGE Integrin beta 1 / CD29 antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat-2 whole cell lysate/extract 7.5% SDS-PAGE Integrin beta 1 / CD29 antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunoprecipitation of Integrin beta 1 / CD29 protein from HeLa whole cell extracts using 5 μg of Integrin beta 1 / CD29 antibody . Western blot analysis was performed using Integrin beta 1 / CD29 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Product Name | Integrin beta 1 / CD29 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Integrin beta 1Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Integrin beta 1 / CD29. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PIPWB |
| WB:1:500~1:1000 IP:1:100~1:500 IHC-P:1:50~1:100 *Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Cat, Pig, Chicken, Sheep, BovineXenopus laevis (100%), Cat (100%), Pig (100%), Chicken (100%), Sheep (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | GPIIA VLA-BETA ITGB1 VLAB integrin beta 1 (fibronectin receptor beta polypeptide antigen CD29 includes MDF2 MSK12) MDF2 FNRB CD29 MSK12 |
|---|---|
| Cellular Localization | Cell membrane, Cleavage furrow, Melanosome |
Application
Wild-type (WT) and Integrin beta 1 / CD29 knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Application
Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein by western blot analysis. A. 30 µg A431 whole cell lysate/extract B. 30 µg HeLa whole cell lysate/extract 7.5% SDS-PAGE Integrin beta 1 / CD29 antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein by western blot analysis. A. 30 µg U87-MG whole cell lysate/extract B. 30 µg SK-N-SH whole cell lysate/extract C. 30 µg IMR32 whole cell lysate/extract D. 30 µg SK-N-AS whole cell lysate/extract 7.5% SDS-PAGE Integrin beta 1 / CD29 antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein on breast cancer by immunohistochemical analysis. Sample: Paraffin-embedded breast cancer. Integrin beta 1 / CD29 antibody dilution: 1:500.
Application
Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein on hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. Integrin beta 1 / CD29 antibody dilution: 1:500.
Application
Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract 7.5% SDS-PAGE Integrin beta 1 / CD29 antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein by western blot analysis. A. 30 µg NIH-3T3 whole cell lysate/extract B. 30 µg BCL-1 whole cell lysate/extract C. 30 µg Raw264.7 whole cell lysate/extract D. 30 µg C2C12 whole cell lysate/extract 7.5% SDS-PAGE Integrin beta 1 / CD29 antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat-2 whole cell lysate/extract 7.5% SDS-PAGE Integrin beta 1 / CD29 antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunoprecipitation of Integrin beta 1 / CD29 protein from HeLa whole cell extracts using 5 μg of Integrin beta 1 / CD29 antibody . Western blot analysis was performed using Integrin beta 1 / CD29 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Positive Control | A431 , HeLa , U87-MG , SK-N-SH , IMR32 , SK-N-AS , Neuro 2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , PC-12 , Rat2 , *J82 |
|---|---|
| Application Notes | WB:1:500~1:1000 IP:1:100~1:500 IHC-P:1:50~1:100 *Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
