Application
Western blot analysis of extracts from HUVEC cells using Integrin beta 3 (Phospho-Tyr785) Antibody and the same antibody preincubated with blocking peptide .Application
Western blot analysis of extracts from HUVEC cells using Integrin b3(Phospho-Tyr785) Antibody and the same antibody preincubated with blocking peptide .| Product Name | Integrin beta 3 (phospho Tyr785) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Integrin beta 3 (Phospho Tyr785)Integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 785 (I-T-Y(p)-R-G) derived from Human Integrin beta 3. |
| Modification | p-Tyr785 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CD61 GP3A GPIIIa ITGB3 integrin beta-3 platelet glycoprotein IIIa platelet membrane glycoprotein IIIa integrin beta 3 (platelet glycoprotein IIIa antigen CD61) |
|---|---|
| Target | Integrin beta 3 (Phospho Tyr785) |
| Molecular Weight(MW) | 110 (note) |
Application
Western blot analysis of extracts from HUVEC cells using Integrin beta 3 (Phospho-Tyr785) Antibody and the same antibody preincubated with blocking peptide .
Application
Western blot analysis of extracts from HUVEC cells using Integrin b3(Phospho-Tyr785) Antibody and the same antibody preincubated with blocking peptide .| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
