Application
Sample (30 ug of whole cell lysate) A: Raji 7.5% SDS PAGE diluted at 1:1000Application
IP3KC antibody [N3C2], Internal detects IP3KC protein at nucleus by immunofluorescent analysis. Sample: RD cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IP3KC protein stained by IP3KC antibody [N3C2], Internal diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | IP3KC antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IP3KC (inositol 1,4,5-trisphosphate 3-kinase C)Inositol 1,4,5-trisphosphate 3-kinase (ITPK) catalyzes the phosphorylation of Ins(1,4,5)P3 to Ins(1,3,4,5)P4, both of which are modulators of calcium homeostasis. ITPK isoforms include ITPKA (MIM 147521), ITPKB (MIM 147522), and ITPKC, all of which contain a conserved catalytic unit in their C termini, but have unique N-terminal sequences and tissue distributions.[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human IP3KC. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (90%), Rat (88%), Bovine (89%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | IP3-3KC antibody IP3KC antibody ITPKC antibody "inositol 1 4 5-trisphosphate 3-kinase C antibody" IP3 3-kinase C antibody InsP 3 kinase C antibody IP3K C antibody inositol-trisphosphate 3-kinase C antibody insP 3-kinase C antibody |
|---|---|
| Molecular Weight(MW) | 75 kDa (note) |
| Cellular Localization | Nucleus , Cytoplasm |
Application
Sample (30 ug of whole cell lysate) A: Raji 7.5% SDS PAGE diluted at 1:1000
Application
IP3KC antibody [N3C2], Internal detects IP3KC protein at nucleus by immunofluorescent analysis. Sample: RD cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IP3KC protein stained by IP3KC antibody [N3C2], Internal diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
