Application
Western Blot analysis of THP-1 (THP) and HeLa (HL) cell lysates probed with Rabbit anti Human IRAKApplication
Immunocytochemical staining of HeLa cells with Rabbit anti Human IRAK| Product Name | IRAK antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal to IRAKNuclear factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFa and LPS. A serine/threonine protein kinase associated with IL-1 receptor (IRAK) and its homologue mouse pelle-like protein kinase (mPLK) were identified recently (1,2). IRAK is associated with the IL-1receptor subunits IL-1RI and IL-1RAcP after IL-1 binding and serves as a signaling molecule to mediate IL-1response (3). IRAK mediates a signaling cascade leading to NF-kB activation by members in IL-1 family including IL-1 and a novel cytokine IL-18 (also termed IGIF) (1,4). |
| Immunogen | Peptide: C-terminus of human IRAK |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| For WB: Use at a dilution of 1:1,000 - 1:2,000. For IP: Use at a dilution of 2 - 4 μg per sample. An 80 kDa band should be detectedin lysates (THP-1 or HeLa whole cell) from non-activated cells. Optimal concentrations/dilutions should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | IRAK1 IRAK 3654 P51617 300283 pelle |
|---|---|
| Target | IRAK |
| Tissue Specificity | Human IRAK |
Application
Western Blot analysis of THP-1 (THP) and HeLa (HL) cell lysates probed with Rabbit anti Human IRAK
Application
Immunocytochemical staining of HeLa cells with Rabbit anti Human IRAK| Positive Control | THP-1 or HeLa whole cell lysate |
|---|---|
| Application Notes | For WB: Use at a dilution of 1:1,000 - 1:2,000. For IP: Use at a dilution of 2 - 4 μg per sample. An 80 kDa band should be detectedin lysates (THP-1 or HeLa whole cell) from non-activated cells. Optimal concentrations/dilutions should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Upon Receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, no preservative added |
