Application
Western blot analysis of IRAK-4 in 30 ugs of NIH 3T3 cell lysate at 1:500 dilution.| Product Name | IRAK4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to IRAK4Interleukin 1 (IL1) and lipopolysaccharide (LPS) induces cellular responses through IL1 receptor (IL1R) and Toll like receptors (TLR). IL1R associated kinases (IRAK, IRAK2, and IRAKM) regulate the activation of NF-kB and MAP kinase (MAPK) by IL1R/TLR. A novel member in the IRAK/Pelle family was recently identified and designated IRAK4. Overexpression of IRAK4 activates NF-kB and MAPK pathways. IRAK4 interacts with and phosphorylates IRAK1. IRAK4 deficient animals are completely resistant to the challenge with LPS. Animals and humans lacking IRAK4 are impaired in their responses to viral and bacterial challenges. Members in IRAK/Pelle family play a central role in IL1R/TLR mediated inflammatory responses and in innate immunity. |
| Immunogen | Synthetic peptide, corresponding to amino acids 38-50 (IKKPSGDDRYNQ) and 120-136 (VAQTHGPCQEKDRTSVM) of mouse IRAK4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a dilution of 1/500 - 1/1000. Detects a band of approximately 51 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Synonyms | 8430405M07Rik 9330209D03Rik IRAK-4 NY-REN-64 IRAK 4 NY REN 64 IRAK4 NYREN64 |
|---|---|
| Target | IRAK4 |
| Cellular Localization | Cell Membrane |
Application
Western blot analysis of IRAK-4 in 30 ugs of NIH 3T3 cell lysate at 1:500 dilution.| Positive Control | NIH 3T3 cell lysate |
|---|---|
| Application Notes | WB: Use at a dilution of 1/500 - 1/1000. Detects a band of approximately 51 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.02% Sodium Azide; Constituents: PBS |
