Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using IRF9 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using IRF9 antibody at 1:250 dilution.Application
Sample (30 µg of whole cell lysate) A: A549 B: HeLa 10% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | IRF9 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IRF9 (interferon regulatory factor 9) |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human IRF9. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Pig, Rhesus Monkey, BovineMouse (81%), Pig (87%), Rhesus Monkey (96%), Bovine (84%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | IRF-9 antibody ISGF3 antibody ISGF3G antibody p48 antibody IRF9 antibody interferon regulatory factor 9 antibody ISGF-3 gamma antibody transcriptional regulator ISGF3 subunit gamma antibody ISGF3 p48 subunit antibody "interferon-stimulated transcr |
|---|---|
| Molecular Weight(MW) | 44 kDa (note) (2) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using IRF9 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using IRF9 antibody at 1:250 dilution.
Application
Sample (30 µg of whole cell lysate) A: A549 B: HeLa 10% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | A549 , HeLa , HepG2 , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
