Application
Western blot analysis of IRS1 (pS1101) expression in HepG2 insulin-treated (A) whole cell lysates.Application
Immunohistochemical analysis of IRS1 (pS1101) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Product Name | IRS1 (phospho Ser1101) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IRS1 (phospho Ser1101)This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq, Nov 2009] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human IRS1. The exact sequence is proprietary. |
| Modification | p-Ser1101 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HIRS-1 insulin receptor substrate 1 IRS1 |
|---|---|
| Target | IRS1 (phospho Ser1101) |
| Tissue Specificity | Recognizes endogenous levels of IRS1 (pS1101) protein. |
Application
Western blot analysis of IRS1 (pS1101) expression in HepG2 insulin-treated (A) whole cell lysates.
Application
Immunohistochemical analysis of IRS1 (pS1101) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Application Notes | WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 °C. Upon delivery aliquot and store at -20 °C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide. |
