Application
Western blot analysis of extract from 293 cells untreated or treated with insulin using IRS-1 (Phospho-Ser312) antibody .| Product Name | IRS1 (phospho Ser312) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IRS-1(Phospho Ser312)May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit |
| Immunogen | Peptide sequence around phosphorylation site of serine 312 (A-T-S(p)-P-A) derived from Human IRS-1. |
| Modification | p-Ser312 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HIRS-1 IRS1 IRS-1 insulin receptor substrate 1 |
|---|---|
| Target | IRS-1(Phospho Ser312) |
| Molecular Weight(MW) | 180 (note) |
Application
Western blot analysis of extract from 293 cells untreated or treated with insulin using IRS-1 (Phospho-Ser312) antibody .| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
