Rabbit polyclonal antibody to IRS1 (phospho Ser639)This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq, Nov 2009]
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human IRS1 around the phosphorylation site of serine 639.
Modification
p-Ser639
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
IHC-PWB
WB: 1:500-1:1000 IHC: 1:50-1:100:
Species Reactivity
HumanMouseRat
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
Purification
Affinity purified
Target Information
Gene Synonyms
IRS1 HIRS-1 insulin receptor substrate 1
Target
IRS1 (phospho Ser639)
Tissue Specificity
This antibody detects endogenous levels of IRS1 only when phosphorylated at serine 639.
Application
Application
IHC-P analysis of human breast carcinoma tissue using IRS1 (phospho Ser639) antibody.
Application
WB analysis of lysates from untreated and serum-treated 293 cells using IRS1 (phospho Ser639) antibody.
Application Notes
WB: 1:500-1:1000 IHC: 1:50-1:100:
Additional Information
Form
Liquid
Storage Instructions
Aliquot and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles.
Storage Buffer
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.