Application
Immunofluorescence staining of methanol-fixed Hela cells using IRS-1 Antibody .Application
Western blot analysis of extracts from HT29 and 3T3 cells using IRS-1 Antibody .Application
Western blot analysis of extracts from HT29 and 3T3 cells using IRS-1(Ab-639) Antibody| Product Name | IRS1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IRS-1May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit |
| Immunogen | Peptide sequence around aa.637~641 (P-K-S-V-S) derived from Human IRS-1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HIRS-1 IRS1 IRS-1 insulin receptor substrate 1 |
|---|---|
| Target | IRS-1 |
| Molecular Weight(MW) | 180 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using IRS-1 Antibody .
Application
Western blot analysis of extracts from HT29 and 3T3 cells using IRS-1 Antibody .
Application
Western blot analysis of extracts from HT29 and 3T3 cells using IRS-1(Ab-639) Antibody| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
