Application
ISCU antibody [N1C3] detects ISCU protein at cytosol on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. ISCU antibody [N1C3] dilution: 1:500.Application
Immunofluorescence analysis of methanol-fixed HeLa, using ISCU antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using ISCU antibody at 1:500 dilution.Application
Sample (30 µg of whole cell lysate) A: HeLa 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | ISCU antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ISCU (iron-sulfur cluster scaffold homolog (E. coli))Iron-sulfur (Fe-S) clusters are necessary for several mitochondrial enzymes and other subcellular compartment proteins. They contain sulfur and iron, and are created via several steps that include cysteine desulfurases, iron donors, chaperones, and scaffold proteins. This gene encodes the two isomeric forms, ISCU1 and ISCU2, of the Fe-S cluster scaffold protein. Mutations in this gene have been found in patients with myopathy with severe exercise intolerance and myoglobinuria. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ISCU. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Rhesus Monkey, BovineRat (97%), Rhesus Monkey (100%), Bovine (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 2310020H20Rik antibody HML antibody ISU2 antibody MGC74517 antibody NIFU antibody NIFUN antibody hnifU antibody ISCU antibody nifU-like N-terminal domain-containing protein antibody "iron-sulfur cluster assembly enzyme ISCU mitochondrial antibody |
|---|---|
| Molecular Weight(MW) | 18 kDa (note) (2) |
| Cellular Localization | Isoform 1: Mitochondrion , Isoform 2: Cytoplasm , Nucleus |
Application
ISCU antibody [N1C3] detects ISCU protein at cytosol on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. ISCU antibody [N1C3] dilution: 1:500.
Application
Immunofluorescence analysis of methanol-fixed HeLa, using ISCU antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using ISCU antibody at 1:500 dilution.
Application
Sample (30 µg of whole cell lysate) A: HeLa 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | A549 , HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
