Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using ISG15 antibody antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: HeLa 15% SDS PAGE diluted at 1:2000Application
Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with ISG15 antibody diluted at 1:2000.Application
ISG15 antibody detects ISG15 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde for 10 min. Green: ISG15 protein stained by ISG15 antibody diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | ISG15 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ISG15 (ISG15 ubiquitin-like modifier)ISG15 is a ubiquitin-like protein that becomes conjugated to many cellular proteins upon activation by interferon-alpha (IFNA; MIM 147660) and -beta (IFNB; MIM 147640) (Zhao et al., 2005 [PubMed 16009940]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ISG15. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanRat |
| Predicted Reactivity | Rhesus MonkeyRhesus Monkey (90%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | G1P2 antibody IFI15 antibody IP17 antibody UCRP antibody hUCRP antibody ISG15 antibody interferon-induced 15 kDa protein antibody interferon-induced 17-kDa/15-kDa protein antibody ubiquitin-like protein ISG15 antibody "interferon alpha-inducible |
|---|---|
| Molecular Weight(MW) | 18 kDa (note) (2) |
| Cellular Localization | Cytoplasm , Secreted |
Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using ISG15 antibody antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: HeLa 15% SDS PAGE diluted at 1:2000
Application
Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with ISG15 antibody diluted at 1:2000.
Application
ISG15 antibody detects ISG15 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde for 10 min. Green: ISG15 protein stained by ISG15 antibody diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | HeLa , rat spleen |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
