Application
SH-SY5Y whole cell and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Islet 1 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Islet 1 antibody detects Islet 1 protein by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: Islet 1 protein stained by Islet 1 antibody diluted at 1:250. Red: Protein kinase C alpha staining. Blue: Fluoroshield with DAPI .Application
Islet 1 antibody detects Islet 1 protein at nucleus by immunofluorescent analysis. Sample: SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Islet 1 protein stained by Islet 1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Islet 1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Islet 1 antibody detects Islet 1 protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. Islet 1 antibody dilution: 1:100.| Product Name | Islet 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Islet 1 (ISL LIM homeobox 1)This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Islet 1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-FIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Japanese medaka, Xenopus laevis, Chicken, BovineZebrafish (97%), Japanese medaka (97%), Xenopus laevis (96%), Chicken (99%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ISLET1 antibody Isl-1 antibody ISL1 antibody insulin gene enhancer protein ISL-1 antibody "ISL1 transcription factor LIM/homeodomain antibody" islet-1 antibody ISL LIM homeobox 1 antibody |
|---|---|
| Molecular Weight(MW) | 39 kDa (note) |
| Cellular Localization | Nucleus |
Application
SH-SY5Y whole cell and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Islet 1 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Islet 1 antibody detects Islet 1 protein by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: Islet 1 protein stained by Islet 1 antibody diluted at 1:250. Red: Protein kinase C alpha staining. Blue: Fluoroshield with DAPI .
Application
Islet 1 antibody detects Islet 1 protein at nucleus by immunofluorescent analysis. Sample: SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Islet 1 protein stained by Islet 1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Islet 1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Islet 1 antibody detects Islet 1 protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. Islet 1 antibody dilution: 1:100.| Positive Control | K562 whole cell extract , K562 nuclear extract , jurkat whole cell extract , jurkat nuclear extract , PC-12 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
