Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using ITCH (Phospho-Tyr420) antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | ITCH (phospho Tyr420) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ITCH (phospho Tyr420)This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012] |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 420(F-I-Y(p)-G-N) derived from Human ITCH . |
| Modification | p-Tyr420 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-P |
| IHC: 1:50 - 1:100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | NAPP1 itchy E3 ubiquitin protein ligase ITCH AIF4 AIP4 dJ468O1.1 |
|---|---|
| Target | ITCH (phospho Tyr420) |
| Tissue Specificity | The antibody detects endogenous levels of ITCH only when phosphorylated at tyrosine 420. |
Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using ITCH (Phospho-Tyr420) antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | IHC: 1:50 - 1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20˚C/1 year |
| Storage Buffer | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
