Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with SPAG9 antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of SPAG9 (arrow) using rabbit polyclonal SPAG9 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SPAG9 gene (Lane 2)| Product Name | JLP / SPAG9 antibody, Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to JLP (SPAG9) (Internal)Extracellular signals are transduced into cells through mitogen-activated protein kinases. The structural organization of these kinases into specific signaling domains is facilitated by scaffolding proteins involved in closely tethering different kinases so that successive phosphorylation events can occur. The protein encoded by this gene is a scaffolding protein that brings together mitogen-activated protein kinases and their transcription factor targets for the activation of specific signaling pathways. This gene which is abundantly expressed in testicular haploid germ cells encodes a protein that is recognized by sperm-agglutinating antibodies and implicated in infertility. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | The immunogen corresponding to a region within amino acids 603 and 632 of human SPAG9. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| Recommended Starting Dilutions: ForWB: Use at a dilution of 1:1000. For IHC-P: Use at a dilution of 1:10-50. Optimal dilutions should be determined experimentally by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Protein highly expressed in testis JIP-4 Human lung cancer protein 6 SPAG9 SYD1 Cjunaminoterminal kinaseinteracting protein 4 JNK-associated leucine-zipper protein Sunday driver 1 JNKassociated leucinezipper protein JIP4 Cancer/testis antigen Su |
|---|---|
| Target | JLP (SPAG9) |
| Molecular Weight(MW) | 146.205 kDa (note) |
| Cellular Localization | Perinuclear distribution in response to stress signals such as UV radiation. Associated with the plasma membrane of the acrosomal compartment and also localizes in the acrosome matrix. |
Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with SPAG9 antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of SPAG9 (arrow) using rabbit polyclonal SPAG9 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SPAG9 gene (Lane 2)| Application Notes | Recommended Starting Dilutions: ForWB: Use at a dilution of 1:1000. For IHC-P: Use at a dilution of 1:10-50. Optimal dilutions should be determined experimentally by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
