Application
Immunohistochemistry of JMJD1A in rat liver tissue with JMJD1A antibody at 5 ug/ml.Application
Western blot analysis of JMJD1A in mouse liver tissue lysate with JMJD1A antibody at (A) 1 and (B) 2 ug/ml.Application
Immunofluorescence of JMJD1A in Rat Liver cells with JMJD1A antibody at 20 ug/mL.| Product Name | JMJD1A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to JMJD1AThe jumonji domain containing 1A protein (JMJD1A) was originally discovered as a testes specific gene, but has been found to be expressed in numerous tissues. JMJD1A is a histone demethylase and specifically demethylates mono- and dimethyl-H3K9. It has also been found to be a novel interaction partner with ER71, a transcription factor expressed in the testes of adult mice and during embryogenesis. JMJD1A is also a downstream gene of STAT3, a protein that has been found to be important in the maintenance of mouse embryonic stem (mES) cells, and decreased JMJD1A expression correlated with the differentiation of cultured mES cells following the removal of the cytokine LIF. These findings suggest that JMJD1A might be a critical signaling molecule underlying the maintenance of pluripotency in embryonic stem cells. At least two isoforms of JMJD1A are known to exist. This antibody will not cross-react with JMJD1B or JMJD1C. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-PWB |
| ICC/IF:1:200~1:500 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | JMJD1A TSGA JHDM2A Lysinespecific demethylase3A KDM3A Lysine-specific demethylase 3A JHMD2A Lysinespecific demethylase 3A Lysine specific demethylase 3A |
|---|---|
| Target | JMJD1A |
| Tissue Specificity | This antibody is specific for JMJD1A |
Application
Immunohistochemistry of JMJD1A in rat liver tissue with JMJD1A antibody at 5 ug/ml.
Application
Western blot analysis of JMJD1A in mouse liver tissue lysate with JMJD1A antibody at (A) 1 and (B) 2 ug/ml.
Application
Immunofluorescence of JMJD1A in Rat Liver cells with JMJD1A antibody at 20 ug/mL.| Positive Control | Blocking Peptide: |
|---|---|
| Application Notes | ICC/IF:1:200~1:500 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.02% sodium azide |
