Application
Western blot analysis of extracts from 293 cells using SAPK/JNK(Phospho-Thr183) Antibody (Lane 2) and the same antibody preincubated with blocking peptide(Lane1).Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SAPK/JNK(Phospho-Thr183) Antibody (left) or the same antibody preincubated with blocking peptide(right).IHC
A, Hematoxylin and eosin staining (scale bar: 20 μm). B, Masson's trichrome staining (scale bar: 20 μm). C–F, Immunohistochemical staining to detect Hsp70 (C, scale bar: 20 μm), p-Akt (D, scale bar: 20 μm), p-JNK (E, scale bar: 20 μm), and p-ERK (F, scale bar: 20 μm) expression.WB
A–E, Western blotting evaluations of Hsp70 (A), cleaved caspase-3 (B), p-Akt and Akt (C), p-JNK and JNK (D), and p-ERK and ERK (E) levels in the myocardium. Previous| Product Name | JNK (phospho Thr183) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to JNK (phospho Thr183)Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells. |
| Immunogen | Peptide sequence around phosphorylation site of threonine 183 (M-M-T(p)-P-Y) derived from Human SAPK/JNK. |
| Modification | p-Thr183 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Cocktail |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ |
| Purification | Affinity purified |
| Gene Synonyms | JNK-55 JNK2 JNK2A JNK2ALPHA JNK2B JNK2BETA PRKM9 SAPK p54a p54aSAPK MAPK9 stress-activated protein kinase 1a MAP kinase 9 stress-activated protein kinase JNK2 Jun kinase MAPK 9 c-Jun N-terminal kinase 2 c-Jun kinase 2 mitogen-activated p |
|---|---|
| Target | JNK (phospho Thr183) |
| Molecular Weight(MW) | 46, 54 (note) |
Application
Western blot analysis of extracts from 293 cells using SAPK/JNK(Phospho-Thr183) Antibody (Lane 2) and the same antibody preincubated with blocking peptide(Lane1).
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SAPK/JNK(Phospho-Thr183) Antibody (left) or the same antibody preincubated with blocking peptide(right).
IHC
A, Hematoxylin and eosin staining (scale bar: 20 μm). B, Masson's trichrome staining (scale bar: 20 μm). C–F, Immunohistochemical staining to detect Hsp70 (C, scale bar: 20 μm), p-Akt (D, scale bar: 20 μm), p-JNK (E, scale bar: 20 μm), and p-ERK (F, scale bar: 20 μm) expression.
WB
A–E, Western blotting evaluations of Hsp70 (A), cleaved caspase-3 (B), p-Akt and Akt (C), p-JNK and JNK (D), and p-ERK and ERK (E) levels in the myocardium. Previous| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
