Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SAPK/JNK Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from Hela and MCF cells using SAPK/JNK Antibody .| Product Name | JNK antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to JNKResponds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells. |
| Immunogen | Peptide sequence around aa.181~185 (M-M-T-P-Y) derived from Human SAPK/JNK. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | JNK-55 JNK2 JNK2A JNK2ALPHA JNK2B JNK2BETA PRKM9 SAPK p54a p54aSAPK MAPK9 stress-activated protein kinase 1a MAP kinase 9 stress-activated protein kinase JNK2 Jun kinase MAPK 9 c-Jun N-terminal kinase 2 c- |
|---|---|
| Target | JNK |
| Molecular Weight(MW) | 46, 54 (note) |
| Tissue Specificity | predicted to detect the JNK1/2/3 protein forms base on the 100% sequence homology (46kd for jnk1, 55kd for jnk2/3) |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SAPK/JNK Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from Hela and MCF cells using SAPK/JNK Antibody .| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
