Application
Western blot analysis of SAPK/JNK expression in LOVO whole cell lysates,The lane on the left is treated with the antigen-specific peptide.WB
(A) Expression of MAPKs and NF-κB in the lung tissues.WB
A–E, Western blotting evaluations of Hsp70 (A), cleaved caspase-3 (B), p-Akt and Akt (C), p-JNK and JNK (D), and p-ERK and ERK (E) levels in the myocardium.| Product Name | JNK antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to JNKThe protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] |
| Immunogen | A synthesized peptide derived from human SAPK/JNK |
| Isotype | IgG |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ |
| Purification | Affinity purified |
| Gene Synonyms | PRKM8 JNK-46 mitogen-activated protein kinase 8 SAPK1c JNK1A2 SAPK1 MAPK8 JNK1 JNK21B1/2 JNK |
|---|---|
| Target | JNK |
| Tissue Specificity | MAPK8 |
Application
Western blot analysis of SAPK/JNK expression in LOVO whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
WB
(A) Expression of MAPKs and NF-κB in the lung tissues.
WB
A–E, Western blotting evaluations of Hsp70 (A), cleaved caspase-3 (B), p-Akt and Akt (C), p-JNK and JNK (D), and p-ERK and ERK (E) levels in the myocardium.| Application Notes | WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
