Application
Western blot analysis of JUNB (pS259) expression in HeLa (A); MCF7 (B) whole cell lysates.Application
Immunohistochemical analysis of JUNB (pS259) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Product Name | JunB (phospho Ser259) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to JunB (phospho Ser259) |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human JUNB (pS259). The exact sequence is proprietary. |
| Modification | p-Ser259 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | JUNB jun B proto-oncogene AP-1 |
|---|---|
| Target | JunB (phospho Ser259) |
| Tissue Specificity | Recognizes endogenous levels of JUNB (pS259) protein. |
Application
Western blot analysis of JUNB (pS259) expression in HeLa (A); MCF7 (B) whole cell lysates.
Application
Immunohistochemical analysis of JUNB (pS259) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Application Notes | WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 °C. Upon delivery aliquot and store at -20 °C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide. |
