Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using JunB (Phospho-Ser259) Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from HT29 cells untreated or treated with EGF using JunB (Phospho-Ser259) Antibody| Product Name | JunB (phospho Ser259) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to JunB (Phospho Ser259)Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'. |
| Immunogen | Peptide sequence around phosphorylation site of serine 259 (P-V-S(p)-P-I) derived from Human JunB. |
| Modification | p-Ser259 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AP-1 JUNB transcription factor jun-B activator protein 1 jun B proto-oncogene |
|---|---|
| Target | JunB (Phospho Ser259) |
| Molecular Weight(MW) | 43 (note) |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using JunB (Phospho-Ser259) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from HT29 cells untreated or treated with EGF using JunB (Phospho-Ser259) Antibody| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
