Application
Immunofluorescence staining of methanol-fixed Hela cells using JunB (Phospho-Ser79) AntibodyApplication
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using JunB (Phospho-Ser79) Antibody (left) or the same antibody preincubated with blocking peptide(right).| Product Name | JunB (phospho Ser79) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to JunB (Phospho Ser79)Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'. |
| Immunogen | Peptide sequence around phosphorylation site of serine 79 (G-A-S(p)-L-K) derived from Human JunB. |
| Modification | p-Ser79 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-P |
| Suggested starting dilutions are as follows. IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AP-1 JUNB transcription factor jun-B activator protein 1 jun B proto-oncogene |
|---|---|
| Target | JunB (Phospho Ser79) |
| Molecular Weight(MW) | 43 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using JunB (Phospho-Ser79) Antibody
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using JunB (Phospho-Ser79) Antibody (left) or the same antibody preincubated with blocking peptide(right).| Application Notes | Suggested starting dilutions are as follows. IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
