Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using JunD (Phospho-Ser255) Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from 293 cells using JunD (Phospho-Ser255) Antibody (Lane 2) and the same antibody preincubated with blocking peptide (Lane1)| Product Name | JunD (phospho Ser255) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to JunD (Phospho Ser255)Binds an AP-1 site and upon cotransfection stimulates the activity of a promoter that bears an AP-1 site. |
| Immunogen | Peptide sequence around phosphorylation site of serine 255 (G-E-S(p)-P-P) derived from Human JunD. |
| Modification | p-Ser255 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AP-1 JUND JunD-FL isoform transcription factor jun-D activator protein 1 jun D proto-oncogene |
|---|---|
| Target | JunD (Phospho Ser255) |
| Molecular Weight(MW) | 38 (note) |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using JunD (Phospho-Ser255) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from 293 cells using JunD (Phospho-Ser255) Antibody (Lane 2) and the same antibody preincubated with blocking peptide (Lane1)| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
