Application
Immunofluorescence staining of methanol-fixed Hela cells using JunD Antibody .Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using JunD AntibodyApplication
Western blot analysis of extracts from HT29 and 293 cells using JunD Antibody .Application
Western blot analysis of extracts from HT29 and 293 cells using JunD(Ab-255) Antibody| Product Name | JunD antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to JunDBinds an AP-1 site and upon cotransfection stimulates the activity of a promoter that bears an AP-1 site. |
| Immunogen | Peptide sequence around aa. 253~257 (G-E-S-P-P) derived from Human JunD. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AP-1 JUND JunD-FL isoform transcription factor jun-D activator protein 1 jun D proto-oncogene |
|---|---|
| Target | JunD |
| Molecular Weight(MW) | 38 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using JunD Antibody .
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using JunD Antibody
Application
Western blot analysis of extracts from HT29 and 293 cells using JunD Antibody .
Application
Western blot analysis of extracts from HT29 and 293 cells using JunD(Ab-255) Antibody| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
