Application
Immunofluorescence analysis of methanol-fixed HCT116, using Kallistatin antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Kallistatin antibody at 1:250 dilution.Application
Sample (30 ug of whole cell lysate) A: HCT116 10% SDS PAGE diluted at 1:1000| Product Name | Kallistatin antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Kallistatin (serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4)Inhibits human amidolytic and kininogenase activities of tissue kallikrein. Inhibition is achieved by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and generation of a small C-terminal fragment of the inhibitor due to cleavage at the reactive site by tissue kallikrein. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Kallistatin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | KAL antibody KLST antibody KST antibody PI4 antibody kallistatin antibody SERPINA4 antibody serpin A4 antibody kallikrein inhibitor antibody "serine (or cysteine) proteinase inhibitor clade A (alpha-1 antiproteinase antitrypsin) member 4 antibod |
|---|---|
| Molecular Weight(MW) | 49 kDa (note) (4) |
| Cellular Localization | Secreted |
Application
Immunofluorescence analysis of methanol-fixed HCT116, using Kallistatin antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Kallistatin antibody at 1:250 dilution.
Application
Sample (30 ug of whole cell lysate) A: HCT116 10% SDS PAGE diluted at 1:1000| Positive Control | HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
