Application
Western blot analysis on HeLa cell lysates using anti-Phospho-TIF1-beta (pS824) RabMAb (cat. #). Cells were either (A) untreated (B) treated with Gamma irradiation.| Product Name | KAP1 (phospho Ser824) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit monoclonal antibody [EPR5248] to TIF1-beta (phospho Ser824)TIF1-beta is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. TIF1-beta mediates transcriptional control by interacting with the Kruppel-associated box repression domain found in many transcription factors. This protein localizes to the nucleus and is thought to associate with specific chromatin regions (1). |
| Immunogen | A phospho specific peptide corresponding to residues surrounding Serine 824 of human TIF1-beta was used as an immunogen. This antibody only detects TIF1-beta phosphorylated at Serine 824. |
| Modification | p-Ser824 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | EPR5248 |
| Host Species | Rabbit |
| Tested Applications | IHC-PIPWB |
| For WB: Use at 1:50,000 - 200,000 : |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KAP1 RNF96 TF1B TIF1B |
|---|---|
| Target | TIF1-beta (phospho Ser824) |
| Cellular Localization | Associated with centromeric heterochromatin during cell differentiation through CBX1. |
Application
Western blot analysis on HeLa cell lysates using anti-Phospho-TIF1-beta (pS824) RabMAb (cat. #). Cells were either (A) untreated (B) treated with Gamma irradiation.| Application Notes | For WB: Use at 1:50,000 - 200,000 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
