Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using KNP-I antibody at 1:250 dilution.Application
Sample (30 ug of whole cell lysate) A: A549 B: HepG2 12% SDS PAGE diluted at 1:1000Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using KNP-I antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.| Product Name | KNP-I antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to KNP-I (chromosome 21 open reading frame 33)This gene encodes a potential mitochondrial protein that is a member of the DJ-1/PfpI gene family. This protein is overexpressed in fetal Down syndrome brain. Alternate splicing results in multiple transcript variants. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human KNP-I. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | MonkeyMonkey (97%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ES1 antibody, GT335 antibody, HES1 antibody, KNPH antibody, KNPI antibody, C21orf33 antibody, "human HES1 protein, homolog to E.coli and zebrafish ES1 protein antibody", Keio novel protein I antibody, "ES1 protein homolog, mitochondrial antibody", chromoso |
|---|---|
| Molecular Weight(MW) | 28 kDa (note) (2) |
| Cellular Localization | Mitochondrion |
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using KNP-I antibody at 1:250 dilution.
Application
Sample (30 ug of whole cell lysate) A: A549 B: HepG2 12% SDS PAGE diluted at 1:1000
Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using KNP-I antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.| Positive Control | A549 , HeLa , HepG2 , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
