Application
KRT71 antibody [N3C3] detects KRT71 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: KRT71 protein stained by KRT71 antibody [N3C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Sample (30 ug of whole cell lysate) A: HepG2 7.5% SDS PAGE diluted at 1:5000Application
KRT71 antibody [N3C3] detects KRT71 protein at membrane on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. KRT71 antibody [N3C3] dilution: 1:500.| Product Name | KRT71 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to KRT71 (keratin 71)K6IRS1 belongs to a family of type II keratins that are specifically expressed in the inner root sheath of hair follicles.[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human KRT71. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cat, Sheep, BovineMouse (91%), Rat (91%), Dog (91%), Cat (93%), Sheep (91%), Bovine (91%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | K6IRS1 antibody, KRT6IRS antibody, KRT6IRS1 antibody, MGC119390 antibody, MGC119391 antibody, KRT71 antibody, K71 antibody, type II inner root sheath-specific keratin-K6irs1 antibody, keratin 6 irs antibody, keratin-71 antibody, hK6irs1 antibody, "keratin, |
|---|---|
| Molecular Weight(MW) | 57 kDa (note) |
Application
KRT71 antibody [N3C3] detects KRT71 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: KRT71 protein stained by KRT71 antibody [N3C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Sample (30 ug of whole cell lysate) A: HepG2 7.5% SDS PAGE diluted at 1:5000
Application
KRT71 antibody [N3C3] detects KRT71 protein at membrane on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. KRT71 antibody [N3C3] dilution: 1:500.| Positive Control | HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
