Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Ku70 antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using Ku70 antibody at 1:100 dilution.Application
Sample (30 ug of whole cell lysate) A: Raji 7.5% SDS PAGE diluted at 1:1000| Product Name | Ku70 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Ku70 (X-ray repair complementing defective repair in Chinese hamster cells 6)The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Ku70. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, Chimpanzee, BovineMouse (90%), Rat (91%), Pig (88%), Chimpanzee (100%), Bovine (87%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CTC75 antibody, CTCBF antibody, G22P1 antibody, KU70 antibody, ML8 antibody, TLAA antibody, XRCC6 antibody, CTC box-binding factor 75 kDa subunit antibody, "ATP-dependent DNA helicase II, 70 kDa subunit antibody", ATP-dependent DNA helicase II 70 kDa subun |
|---|---|
| Molecular Weight(MW) | 70 kDa (note) (3) |
| Cellular Localization | Nucleus |
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Ku70 antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using Ku70 antibody at 1:100 dilution.
Application
Sample (30 ug of whole cell lysate) A: Raji 7.5% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , Jurkat , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
