Application
IP analysis was performed with HERG-expressing HEK293 lysates and either Kv11.1 antibody (lane 2) or pre-immune serum (lane 3). The control lysate (lane 1) and precipitates (lanes 2 and 3) were detected by the same antibody. Red arrow indicates the Kv11.1 protein while the black arrow shows the IgG heavy chain.Application
WB analysis of HERG-expressing HEK293 lysates using Kv11.1 antibody at a dilution of 1:200 in the (1) absence or (2) presence of blocking peptide.| Product Name | Kv11.1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Kv11.1voltage gated potassium channel pore forming subunit [RGD, Feb 2006] |
| Immunogen | Peptide (CY)EEL PAGAP ELPQD GPT, corresponding to residues 1122-1137 of rat Kv11.1 (erg1) (Accession O08962). Intracellular, C-terminal part. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| ICC/IF:1:200~1:500 IHC:1:50~1:200 IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ERG1 Kcnh2 potassium voltage-gated channel subfamily H (eag-related) member 2 |
|---|---|
| Target | Kv11.1 |
Application
IP analysis was performed with HERG-expressing HEK293 lysates and either Kv11.1 antibody (lane 2) or pre-immune serum (lane 3). The control lysate (lane 1) and precipitates (lanes 2 and 3) were detected by the same antibody. Red arrow indicates the Kv11.1 protein while the black arrow shows the IgG heavy chain.
Application
WB analysis of HERG-expressing HEK293 lysates using Kv11.1 antibody at a dilution of 1:200 in the (1) absence or (2) presence of blocking peptide.| Application Notes | ICC/IF:1:200~1:500 IHC:1:50~1:200 IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3. |
