Application
Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft, using Laforin antibody at 1:100 dilution.Application
Laforin antibody [C2C3], C-term detects EPM2A protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: EPM2A protein stained by Laforin antibody [C2C3], C-term diluted at 1:500. Blue: Hoechst 33343 staining.Application
Sample(30 μg of whole cell lysate) A:Raji 12% SDS PAGE diluted at 1:5000Application
Laforin antibody detects Laforin protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Laforin antibody diluted by 1:3000.| Product Name | Laforin antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Laforin (laforin)This gene encodes a dual-specificity phosphatase that associates with polyribosomes. The encoded protein may be involved in the regulation of glycogen metabolism. Mutations in this gene have been associated with myoclonic epilepsy of Lafora. Alternative splicing results in multiple transcript variants. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Laforin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | EPM2 antibody MELF antibody EPM2A antibody lafora PTPase antibody laforin antibody "epilepsy progressive myoclonus type 2 Lafora disease (laforin) antibody" LAFPTPase antibody "epilepsy progressive myoclonus type 2A Lafora disease (laforin) anti |
|---|---|
| Molecular Weight(MW) | 37 kDa (note) |
| Cellular Localization | Cytoplasm , Isoform 1: Endoplasmic reticulum , Cell membrane , Isoform 2: Endoplasmic reticulum , Nucleus |
Application
Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft, using Laforin antibody at 1:100 dilution.
Application
Laforin antibody [C2C3], C-term detects EPM2A protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: EPM2A protein stained by Laforin antibody [C2C3], C-term diluted at 1:500. Blue: Hoechst 33343 staining.
Application
Sample(30 μg of whole cell lysate) A:Raji 12% SDS PAGE diluted at 1:5000
Application
Laforin antibody detects Laforin protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Laforin antibody diluted by 1:3000.| Positive Control | 293T , A431 , HeLa , HepG2 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
