Application
Sample (30 µg of whole cell lysate) A: A431 7.5% SDS PAGE Laminin beta-3 antibody diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of methanol-fixed HeLa, using Laminin beta 3 antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded human lung cancer, using Laminin beta 3 antibody at 1:250 dilution.| Product Name | Laminin beta 3 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Laminin beta 3 (laminin, beta 3)The product encoded by this gene is a laminin that belongs to a family of basement membrane proteins. This protein is a beta subunit laminin, which together with an alpha and a gamma subunit, forms laminin-5. Mutations in this gene cause epidermolysis bullosa junctional Herlitz type, and generalized atrophic benign epidermolysis bullosa, diseases that are characterized by blistering of the skin. Multiple alternatively spliced transcript variants that encode the same protein have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Laminin beta 3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | BovineBovine (80%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BM600-125KDA antibody FLJ99565 antibody LAM5 antibody LAMNB1 antibody LAMB3 antibody kalinin B1 chain antibody laminin B1k chain antibody nicein-125kDa antibody "laminin beta 3 (nicein (125kD) kalinin (140kD) BM600 (125kD)) antibody" kalinin su |
|---|---|
| Molecular Weight(MW) | 130 kDa (note) (3) |
| Cellular Localization | Secreted , extracellular space , extracellular matrix , basement membrane |
Application
Sample (30 µg of whole cell lysate) A: A431 7.5% SDS PAGE Laminin beta-3 antibody diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of methanol-fixed HeLa, using Laminin beta 3 antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded human lung cancer, using Laminin beta 3 antibody at 1:250 dilution.| Positive Control | A431 , H1299 , HeLa , HepG2 , NIH-3T3 , *mouse bone marrow |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
