Rabbit Polyclonal antibody to Langerin (CD207 molecule, langerin)The protein encoded by this gene is expressed only in Langerhans cells which are immature dendritic cells of the epidermis and mucosa. It is localized in the Birbeck granules, organelles present in the cytoplasm of Langerhans cells and consisting of superimposed and zippered membranes. It is a C-type lectin with mannose binding specificity, and it has been proposed that mannose binding by this protein leads to internalization of antigen into Birbeck granules and providing access to a nonclassical antigen-processing pathway. [provided by RefSeq]
Immunogen
Recombinant protein encompassing a sequence within the center region of human Langerin. The exact sequence is proprietary.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
WB
*Optimal dilutions/concentrations should be determined by the researcher.
:
Species Reactivity
Human
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
CLEC4K antibody CD207 antibody Langerhans cell specific c-type lectin antibody "C-type lectin domain family 4 member K antibody" "CD207 antigen langerin antibody" C-type lectin domain family 4 member K antibody "CD207 molecule langerin antibody"
Molecular Weight(MW)
37 kDa (note) (2)
Cellular Localization
Membrane
Application
Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Langerin antibody [N2C3] diluted at 1:1000.
Application
Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE diluted at 1:1000
Positive Control
A549 , HeLa , HepG2
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher.
:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.