Application
LARS2 antibody [N2C1], Internal detects LARS2 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LARS2 protein stained by LARS2 antibody [N2C1], Internal diluted at 1:500. Blue: Hoechst 33342 staining.Application
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using LARS2 antibody at 1:100 dilution.Application
Sample (30 ug of whole cell lysate) A: Molt-4 7.5% SDS PAGE diluted at 1:3000Application
Immunofluorescence analysis of methanol-fixed A431, using LARS2 antibody at 1:200 dilution.| Product Name | LARS2 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to LARS2 (leucyl-tRNA synthetase 2, mitochondrial)This gene encodes a class 1 aminoacyl-tRNA synthetase, mitochondrial leucyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human LARS2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (86%), Rat (89%), Bovine (87%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | KIAA0028 antibody LEURS antibody MGC26121 antibody LARS2 antibody "probable leucyl-tRNA synthetase mitochondrial antibody" "leucine tRNA ligase 2 mitocondrial antibody" leucine translase antibody "leucyl-tRNA synthetase 2 mitochondrial antibody" |
|---|---|
| Molecular Weight(MW) | 102 kDa (note) |
| Cellular Localization | Mitochondrion matrix |
Application
LARS2 antibody [N2C1], Internal detects LARS2 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LARS2 protein stained by LARS2 antibody [N2C1], Internal diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using LARS2 antibody at 1:100 dilution.
Application
Sample (30 ug of whole cell lysate) A: Molt-4 7.5% SDS PAGE diluted at 1:3000
Application
Immunofluorescence analysis of methanol-fixed A431, using LARS2 antibody at 1:200 dilution.| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
