Application
Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with LAT antibody diluted at 1:500.| Product Name | LAT antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to LAT (linker for activation of T cells)The protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human LAT. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 36 kDa phospho-tyrosine adapter protein antibody 36 kDa phospho-tyrosine adaptor protein antibody LAT antibody LAT antibody LAT1 antibody LAT1 antibody linker for activation of T cells antibody linker for activation of T cells transme |
|---|---|
| Target | LAT |
| Molecular Weight(MW) | 28 kDa (note) (4) |
| Cellular Localization | Cell membrane; Single-pass type III membrane protein |
Application
Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with LAT antibody diluted at 1:500.| Positive Control | Jurkat |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
