Application
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using LATS1/2 (Ab-1079/1041) Antibody. The picture on the right is treated with the synthesized peptide.| Product Name | LATS1/2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to LATS1/2Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human LATS1/2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCIHC-P |
| ELISA: 1:40000Optimal dilutions should be determined experimentally by the end user.: |
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| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Serine/threonine-protein kinase k Serine/threonineprotein kinase kpm hwarts/EC 2.7.11.1 LATS1/2 Wartslike kinase Large tumor suppressor homolog 2 Large tumor suppressor homolog 1 WARTS protein kinase Warts-like kinase Large tumor suppressor homolo |
|---|---|
| Target | LATS1/2 |
| Molecular Weight(MW) | 126 kDa (note) |
| Tissue Specificity | LATS1/2 (Ab-1079/1041) Antibody detects endogenous levels of total LATS1/2 protein. |
| Cellular Localization | Localizes to the centrosomes throughout interphase but migrates to the mitotic apparatus, including spindle pole bodies, mitotic spindle, and midbody, during mitosis. |
Application
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using LATS1/2 (Ab-1079/1041) Antibody. The picture on the right is treated with the synthesized peptide.| Application Notes | ELISA: 1:40000Optimal dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 degrees Celcius. Product is stable for at least 1 year. |
| Storage Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
