Application
WB analysis of MOLT lysate using Lck (phospho Tyr394) antibody pre-incubated with or without blocking peptide.| Product Name | Lck (phospho Tyr394) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Lck (phospho Tyr394)This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants, encoding the same protein, have been described. [provided by RefSeq, Jul 2008] |
| Immunogen | The antiserum was produced against synthesized phosphopeptide derived from human Lck around the phosphorylation site of tyrosine 394. |
| Modification | p-Tyr394 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1:500-1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | lymphocyte-specific protein tyrosine kinase LCK p56lck YT16 pp58lck LSK |
|---|---|
| Target | Lck (phospho Tyr394) |
| Tissue Specificity | This antibody detects endogenous levels of Lck only when phosphorylated at tyrosine 394. |
Application
WB analysis of MOLT lysate using Lck (phospho Tyr394) antibody pre-incubated with or without blocking peptide.| Application Notes | WB: 1:500-1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
